96-Well Non-Skirted PCR Plates

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Model Number: NH-94858

Made with virgin medical grade polypropylene or dual component hard-shell design

Ultra-thin wall thickness for excellent thermal transfer

RNase/ DNase/ human genomic DNA free, pyrogen free

Wide variety of well formats, skirt designs, and colours to ensure compatibility with most popular PCR instruments

Description

PCR plates are consumable labware used in the polymerase chain reaction (PCR) process. They are designed to hold multiple PCR reactions simultaneously, allowing for high-throughput amplification of DNA or RNA fragments. PCR plates come in various formats and configurations to suit different experimental needs. Here are some key features and characteristics:

  • Well Format: PCR plates typically feature a 96-well format, with each well capable of holding a single PCR reaction. Some plates may also come in 384-well formats, providing higher throughput for large-scale experiments.
  • Material: PCR plates are commonly made from polypropylene, which is compatible with the thermal cycling conditions required for PCR. Some plates may also be made from other plastics such as polyethylene or polycarbonate.
  • Thermal Stability: PCR plates are designed to withstand the temperature fluctuations encountered during PCR cycling, including heating to denature DNA, annealing of primers, and extension of DNA strands.
  • Skirted vs. Non-skirted: PCR plates may have skirted or non-skirted designs. Skirted plates feature raised rims around the wells, providing stability and preventing cross-contamination between wells during sealing. Non-skirted plates have a flat design and are often used with adhesive seals.
  • Barcoding: Some PCR plates come with alphanumeric grid labels or barcode labels, which facilitate sample tracking and identification, especially in high-throughput applications.
  • Optical Clarity: For real-time PCR applications, PCR plates with optically clear wells are available. These plates allow for fluorescence detection during amplification and are compatible with various real-time PCR instruments.
  • Compatibility: PCR plates are designed to fit standard PCR thermal cyclers, ensuring compatibility with common laboratory equipment.
  • Sealing Options: PCR plates can be sealed using various methods, including adhesive seals, heat seals, or cap strips. Effective sealing prevents sample evaporation and contamination during PCR cycling.
  • Sterility: Some PCR plates come pre-sterilized, which is essential for applications requiring high levels of cleanliness and sample integrity.

When selecting PCR plates, it’s essential to consider factors such as well format, material compatibility with PCR reagents, skirted vs. non-skirted design, and any specific requirements for the PCR application being performed. Additionally, quality PCR plates should be free from contaminants that could interfere with PCR reactions and provide reliable and reproducible results.

 

For more information, contact us 01274 965089 or check out our website at www.nicheofficesolutions.co.uk www.nicheofficesolutions.co.uk/niche-nhs

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If you have any additional questions, drop us an email at info@nicheofficesolutions.co.uk 

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