PCR Tubes (Polymerase Chain Reaction)

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Model Number: NH-97593

Brand: Niche Healthcare


Features & Benefits:

  • Made of high quality medical grade polypropylene, autoclavable
  • Ultra thin-wall tubes provide faster heating and cooling
  • High degree of transparency allows for easy observation of samples
  • Flat caps are ideal for Real-Time PCR (qPCR)
  • Unique cap design prevents opening during cycling and incubation stages
  • DNase, RNase, and endotoxin free



  • Fits most standard thermocyclers
  • Can withstand centrifugation of up to 6,000 RCF


PCR Tubes Size Chart

PCR Tubes Size Chart


PCR tubes are small cylindrical containers used in the Polymerase Chain Reaction (PCR) technique, a method widely employed in molecular biology to amplify DNA segments. These tubes are designed to withstand the high temperatures necessary for the PCR process, typically ranging from around 90°C to 100°C during the denaturation step, and 72°C during the annealing and extension steps.

Here are some key features and considerations regarding PCR tubes:

  • Material: PCR tubes are commonly made from polypropylene, a material known for its thermal stability and resistance to chemical reactions. This ensures that the tubes can withstand the temperature fluctuations encountered during the PCR process without deforming or melting.
  • Thin-Walled Design: PCR tubes have thin walls to allow for efficient heat transfer, ensuring rapid heating and cooling of the reaction mixture. This facilitates the thermal cycling required for the denaturation, annealing, and extension steps of PCR.
  • Cap Design: PCR tubes typically come with flat or domed caps that provide a secure seal to prevent evaporation and contamination of the reaction mixture. The caps may feature O-rings or other sealing mechanisms to ensure a tight closure.
  • Compatibility: PCR tubes are compatible with various PCR machines and thermal cyclers available in the market. They are designed to fit securely into the heating block or thermal cycler, ensuring uniform heat distribution across all samples.
  • Volume Capacity: PCR tubes are available in different volume capacities, typically ranging from 0.2 mL to 0.5 mL. The choice of tube volume depends on the specific PCR application and the amount of sample required.
  • Colour Options: PCR tubes may come in different colours, such as clear, white, or assorted colours. Coloured tubes can help with sample identification and organization, particularly in high-throughput PCR applications with multiple samples.
  • Strip Tubes: In addition to individual PCR tubes, PCR strip tubes are also available, where multiple tubes are connected in a single strip. This design allows for easier handling and loading of samples, particularly in high-throughput PCR workflows.
  • Sterility: PCR tubes are available in both sterile and non-sterile formats. Sterile tubes are commonly used in molecular biology and diagnostic applications to prevent contamination of the PCR reaction.

PCR tubes are essential components of PCR assays, providing a reliable and efficient means of amplifying DNA for various downstream applications such as DNA sequencing, genotyping, cloning, and gene expression analysis.


For more information, contact us 01274 965089 or check out our website at www.nicheofficesolutions.co.uk www.nicheofficesolutions.co.uk/niche-nhs

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If you have any additional questions, drop us an email at info@nicheofficesolutions.co.uk 


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